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<!DOCTYPE html>
<html lang="en">
<head>
  <meta charset="utf-8">
  <title>jQuery .eq(-2)</title>
</head>
<body>
  <ul>
    <li>list item 1</li>
    <li>list item 2</li>
    <li>list item 3</li>
    <li>list item 4</li>
    <li>list item 5</li>
  </ul>
  <script src="https://code.jquery.com/jquery-3.7.1.min.js"></script>
  <script>
    $("li").eq(-2).css("background-color", "red");
  </script>
</body>
</html>

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jQuery .eq(-2) — from end (Try It)